mt1  (Alomone Labs)

Journal: The Journal of Headache and Pain

Article Title: Expression of melatonin receptors in trigeminal and sphenopalatine ganglia: potential targets for primary headache disorders

doi: 10.1186/s10194-025-02215-9

Figure Lengend Snippet: Expression of MT1 and MT2 genes in rat TG, SPG and hypothalamus. ( A ) Comparison of MT1 and MT2 mRNA levels in the TG of male and female rats shows that MT1 expression is significantly higher than MT2. ( B ) In both the hypothalamus (HT) and SPG of male rats, MT1 mRNA levels are also significantly higher than MT2. Data are presented as mean ± SEM, statistical significance indicate as *** p < 0.001, ** p < 0.01, * p < 0.05, and n = 6

Article Snippet: MT1 (AMR-031) , 1:200 , Rabbit , Alomone Labs, Jerusalem, Israel , AB_11218959.

Techniques: Expressing, Comparison

Journal: The Journal of Headache and Pain

Article Title: Expression of melatonin receptors in trigeminal and sphenopalatine ganglia: potential targets for primary headache disorders

doi: 10.1186/s10194-025-02215-9

Figure Lengend Snippet: MT1 and MT2 immunoreactivity in TG. ( A ) positive MT1 immunoreactivity was observed in the cytoplasm and nuclei of TG neurons (thick arrows) and in SGCs (arrowheads). ( B ) MT2 immunoreactivity was observed predominantly in the cytoplasm of neurons (thick arrows), in some cases in their nuclei (thick arrows), and in Aδ-fibers (thin arrows). Negative cells are indicated by asterisks in both ( A ) and ( B ). Blue color represents nuclei staining with DAPI. ( C ) Bar graphs show the number of MT1 and MT2 immunoreactive TG neurons in male and female rats. There was no significant difference in MT1 or MT2 protein expression between sexes. However, the number of MT1-immunoreactive cells was approximately twofold higher than that of MT2 in both males and females. Data are presented as the mean ± SEM, n = 6 and * p < 0.05

Article Snippet: MT1 (AMR-031) , 1:200 , Rabbit , Alomone Labs, Jerusalem, Israel , AB_11218959.

Techniques: Staining, Expressing

Journal: The Journal of Headache and Pain

Article Title: Expression of melatonin receptors in trigeminal and sphenopalatine ganglia: potential targets for primary headache disorders

doi: 10.1186/s10194-025-02215-9

Figure Lengend Snippet: Double immunohistochemistry of MT1 with CGRP or RAMP1 in TG. ( A ) CGRP (red fluorescence) was expressed in the cytoplasm of TG neurons (thick arrow) and in C- fibers (arrowhead). ( B ) MT1 (green fluorescence) was expressed in both cytoplasm and nuclei of TG neurons (thick arrow). ( C ) The merged image shows that MT1 was co-localized with CGRP in the cytoplasm of small to medium-sized neurons (yellow, thick arrow). ( D ) RAMP1 (red fluorescence) was expressed in the cytoplasm of medium to large- sized TG neurons (thick arrow) and in Aδ-fibers (arrowhead). ( E ) MT1 (green fluorescence) was expressed in both cytoplasm and nuclei of TG neurons (thick arrow). ( F ) Their co-localization (yellow) is seen in the merged image. MT1 was co-localized with RAMP1 in the cytoplasm of medium to large -sized neurons (thick arrow). Blue represents nuclei staining with DAPI

Article Snippet: MT1 (AMR-031) , 1:200 , Rabbit , Alomone Labs, Jerusalem, Israel , AB_11218959.

Techniques: Immunohistochemistry, Fluorescence, Staining

Journal: The Journal of Headache and Pain

Article Title: Expression of melatonin receptors in trigeminal and sphenopalatine ganglia: potential targets for primary headache disorders

doi: 10.1186/s10194-025-02215-9

Figure Lengend Snippet: Localization of MT1 immunoreactivity in the SPG and its co-localization with VIP and PACAP. ( A , D and G ) MT1 immunoreactivity (green) was observed in both the cytoplasm and nuclei of SPG neurons (thick arrows), as well as in SGCs (arrowheads). ( B and E ) VIP immunoreactivity (red) was detected in the cytoplasm of SPG neurons (thick arrows) and in a few C-fibers (arrowhead). ( C and F ) MT1 was co-localized with VIP in the cytoplasm of some SPG neurons (yellow-orange, thick arrows). D , E and F are higher-magnification views of images A , B and C , respectively. ( H ) PACAP immunoreactivity (red) was found in both the cytoplasm and nuclei of some SPG neurons (thick arrow). ( I ) MT1 was co-localized with PACAP in SPG neurons as shown in merged images (yellow/orange, thick arrow)

Article Snippet: MT1 (AMR-031) , 1:200 , Rabbit , Alomone Labs, Jerusalem, Israel , AB_11218959.

Techniques:

Journal: The Journal of Headache and Pain

Article Title: Expression of melatonin receptors in trigeminal and sphenopalatine ganglia: potential targets for primary headache disorders

doi: 10.1186/s10194-025-02215-9

Figure Lengend Snippet: MT1 and MT2 immunoreactivity in the DRG. ( A ) MT1 expression in DRG was observed in both the cytoplasm and nuclei of neurons (thick arrow) and in SGCs (arrowheads). ( B ) MT2 immunoreactivity was localized in the cytoplasm of neurons and in some cases in their nuclei (thick arrow), and in the Aδ-fibers (thin arrows). The blue color represents nuclei staining with DAPI

Article Snippet: MT1 (AMR-031) , 1:200 , Rabbit , Alomone Labs, Jerusalem, Israel , AB_11218959.

Techniques: Expressing, Staining

Journal: The Journal of Headache and Pain

Article Title: Expression of melatonin receptors in trigeminal and sphenopalatine ganglia: potential targets for primary headache disorders

doi: 10.1186/s10194-025-02215-9

Figure Lengend Snippet: Immunohistochemistry of MT1 expression in the wall of cerebral arteries. ( A and B ) MT1 immunoreactivity (red) was observed in all layers of the basilar artery (thick arrows), i.e., in the adventitia layer and the endothelium as well as in the smooth muscle cells. Green represents the internal elastic lamina, autofluorescence (arrowhead in B ) and blue represents nuclei staining with DAPI

Article Snippet: MT1 (AMR-031) , 1:200 , Rabbit , Alomone Labs, Jerusalem, Israel , AB_11218959.

Techniques: Immunohistochemistry, Expressing, Staining

Journal: The Journal of Neuroscience

Article Title: Selective Enhancement of REM Sleep in Male Rats through Activation of Melatonin MT₁Receptors Located in the Locus Ceruleus Norepinephrine Neurons

doi: 10.1523/jneurosci.0914-23.2024

Figure Lengend Snippet: Figure 3. Immunohistochemical expression of the MT1 receptor in norepinephrine neurons of the LC. A, Schematical representation of the LC taken for the Western blot analysis. B, Immunoblotting with anti-MT1 (1:200) and anti–α-tubulin (aT; 1:10,000) antibodies. Upper bands correspond to the positive blotting from the endogenous control α-tubulin protein whereas the lower bands correspond to the positive blotting from the MT1 protein. MT1 receptors decorated bands of approximately 40 kDa, at their expected molecular weight. The right panel displays the immunoblot with the immunogen peptide. Each lane represents the blotting from one rat. C, Immunoreactivity of MT1 receptors in the LC, 5× (top) and 20× (bottom) show both sides of the LC nucleus expressing MT1 receptors. Scale bar: 250 µm (top), 100 µm (bottom). D, Double immunofluorescence confirmed the expression of MT1 receptors (red) in LC-NE neurons (TH, green). Sections were counterstained with DAPI (blue). Scale bar: 100 µm, 20 µm (expand). E, Percentage of immunoreactivity of neuronal colocalization (yellow), MT1 cellular expression alone (red), and TH neuronal expression alone (green).

Article Snippet: The membranes were incubated (3 h) in a blocking buffer containing 5% nonfat milk in 0.1 M PBS and then rabbit polyclonal anti-MT1 antibodies (AMR-031, conc 1:1,000 in 5% nonfat milk in PBS-Tween 20 0.2% buffer, Alomone Labs) overnight at 4°C.

Techniques: Immunohistochemical staining, Expressing, Western Blot, Control, Molecular Weight